How cells undergo cell division has fascinated many researchers around the world for over a century. Proper orientation and elongation of the mitotic spindle are critical processes for determining the correct positioning of the cytokinetic furrow and is thus essential for determining the relative size and spatial disposition of the resulting daughter cells during development. These processes further ensure that the cell fate determinants are appropriately segregated in the resulting daughter cells during asymmetric cell division, including in the stem cells. In our laboratory, we combine multifaceted approaches of the cell and molecular biology to advance our current understanding in the field of spindle behavior during mitotic progression in human cells and Caenorhabditis elegans embryos.